Nyctanthes arbortristis Linn. (Oleaceae) which is a well known medicinal plan. In order to determine Anti-inflammatory and Analgesic activity of ethanolic extract of root, bark and seed’s of Nyctanthes arbortristis Linn. (Oleaceae) in mice. In order to verify traditional use to evaluate Anti-inflammatory and analgesic activity. Carrageenan induced paw edema test to evaluate Anti-inflammatory activity while Tail immersion and Acetic acid Writhing test is to evaluate analgesic activity in mice. The ethanolic extract of Nyctanthes arbortristis Linn. (oleaceae) 250 mg/kg produced significant reduction in no. of writhing is 8.42 and 46.19% inhibition while 500 mg/kg extract shows no. of writhing is 7.15 and 54.31% inhibition. In tail immersion (250 an 500 mg/kg) after 60 min extract shows 80.23% and 84.0% protection respectively of tail flicking time. From the above study it indicate that ethanolic extract of Nyctanthes arbortristis Linn. (oleaceae) is beneficial
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